GUILLAUME LAMOUR

GUILLAUME LAMOUR

Poste Actuel

Ingénieur de Recherche - Equipe 4

    Research Activities

    My research is at the crossroads of biomaterials  and biophysics. I have developed an expertise in Atomic Force Microscopy (AFM) to study biological materials, from biopolymers to living cells.

     

    • My Ph.D. work (2006–2010, University of Paris Diderot and Paris Descartes, France) focused on studying neuronal growth on modified surfaces. I showed that spatial variations in adhesion energy could dramatically affect cell adhesion and differentiation into neurons. This might help in designing new biocompatible surfaces for nerve regeneration.

    → Publications:

    Lamour et al.  Colloids Surf B 2009

    Lamour et al.  Biomaterials 2010

    Lamour et al.  JBMR-A 2011

    Lamour et al.  J Chem Educ 2011

    Lamour et al.  Macromol Biosci 2015

     

     

     

    • My first postdoc (2011–2016, University of British Columbia, Vancouver, Canada) focused on amyloids. I connected their mechanical properties with the intermolecular interactions in the fibrils. This helps understanding the molecular origins of amyloid diseases (e.g. Parkinson’s, prion diseases) and may help in designing amyloid-based nanomaterials with tailored mechanical properties.

     

    → Publications:

    Lamour et al.  ACS Nano 2014

    Lamour et al.  Source Code Biol Med 2015

    Lamour et al.  Biophys J  2017

    Nassar,…, and Lamour.  J Phys Chem Lett 2018

    Nassar,…, and Lamour.  JACS 2019

     

     

    • In my current work as a Research Engineer (2017-present, University of Evry - Paris Saclay - Genopole grant), I develop new approaches to study the nanomechanics of biomimetic membranes and of living cells, using atomic force microscopy (AFM).   This helps understanding mechanisms underlying endocytosis, vesicle formation, and may open up new research avenues in drug design targeting membrane-cytoskeleton interactions.

    Meanwhile, I collaborate with research groups in France and abroad to develop AFM activities in the Lambe in a variety of projects.  For instance, we have worked with new nanofiber materials exhibiting extraordinary mechanical properties and are currently working on the mechanical properties of disease models of cardiomyocytes.

    → Publications:

    Lamour et al.  Phys Rev X 2020

    Christoff-Tempesta et al.  Nat Nanotechnol. 2021

    Kim et al.  Nano Lett. 2021

    Cho et al.  Nat Commun. 2021

    Liboz et al.  ACS Appl. Mater. Interfaces 2023

    Seffouh et al.  Sci. Rep. 2023

     

     

     [....more coming !]

     

     

    Present and past collaborators:


    • Julia Ortony (Massachusetts Institute of Technology) Nano Lett 2021; Nat. Nanotechnol. 2020
    • Martin Lenz (LPTMS, Universités Paris-Sud and Paris Saclay) Physical Review X 2020;
    • Clément Campillo, Sid Labdi, Juan Pelta (Université d'Evry-Paris SaclayPhysical Review X 2020;
    • Joerg Gsponer (University of British Columbia) JACS 2019; J Phys Chem Lett 2018; Biophys J 2017;...
    • Hongbin Li (UBC) J Phys Chem Lett 2018; JACS 2014; ACS Nano 2014; Biophys J 2012;...
    • Neil Cashman (UBC) ACS Chem Neurosci 2018; J Neurosci 2013
    • Hao Wu (Harvard Medical School) Biophys J 2017
    • Thibault Mayor (UBC) Biophys J 2017
    • Xiaonan Lu (UBC) Int J Food Microbiol 2016
    • Dieter Bromme (UBC) J Biol Chem 2015; J Biol Chem 2013
    • Tuomas Knowles (Cambridge University) Source Code Biol Med 2014
    • Nancy Forde (Simon Fraser University) Biomacromolecules 2014
    • Ahmed Hamraoui (UPMC) Macromol Biosci 2015; J Biomed Mat Res 2011; Biomaterials 2010;...
    • Eric Borguet (Temple University) J Chem Educ 2010; Biomaterials 2010

    Journal Publications

    h-index = 16.
    → Since 2018: > 900 citations.

    • Webpages (including citation profile):

    Google Scholar
    ResearcherID
    

    • Selected publications

    On using AFM to study lipid membranes:

    Lamour G., A. Allard, J. Pelta, S. Labdi, M. Lenz, and C. Campillo. Mapping and Modeling the Nanomechanics of Bare and Protein-Coated Lipid Nanotubes. PHYS REV X. 2020; 10:011031-1–17. doi.org/10.1103/PhysRevX.10.011031

    On using multifrequency AFM to study amyloids:

    – Nassar R., E. Wong, JM. Bui, CK. Yip, HB. Li, J. Gsponer, and G. Lamour. Mechanical anisotropy in GNNQQNY amyloid crystals. J PHYS CHEM LETT. 2018; 9: 4901–09.  doi.org/10.1021/acs.jpclett.8b02027

    On using statistical mechanics and simulations to study amyloids:

    Lamour G., R. Nassar, PHW. Chan, G. Bozkurt, J. Li, JM. Bui, C. Yip, T. Mayor, HB. Li, H. Wu, and J. Gsponer. Mapping the broad structural and mechanical properties of amyloid fibrils. BIOPHYS J. 2017; 112:584–594. doi.org/10.1016/j.bpj.2016.12.036

    On biomaterials surface effects on neuronal growth:

    Lamour G., A. Eftekhari-Bafrooei, E. Borguet, S. Souès, and A. Hamraoui. Neuronal adhesion and differentiation driven by nanoscale surface free-energy gradients. BIOMATERIALS. 2010; 31:3762–71.  doi.org/10.1016/j.biomaterials.2010.01.099 

    On developing technical and software tools:

    Lamour G., JB. Kirkegaard, HB. Li, TPJ. Knowles, and J. Gsponer. Easyworm: an open-source software tool to determine the mechanical properties of worm-like chains. SOURCE CODE BIOL MED. 2014; 9:16.1–16.6.  doi.org/10.1186/1751-0473-9-16

     

    • Publications (full list):

       

    2023

    [26]Seffouh I., M. Bilong, C. Przybylski, N. El Omrani, S. Poyer, G Lamour, M-J Clément, F. Gonnet, RR. Vivès, R. Daniel. Structure and functional impact of glycosaminoglycan modification of HSulf-2 endosulfatase revealed by atomic force microscopy and mass spectrometry. SCI. REP. In press

    [25]Liboz M., A. Allard, M. Malo, G. Lamour, G. Letort, B. Thiébot, S. Labdi, J. Pelta, and C. Campillo. Using adhesive micropatterns and AFM to assess cancer cell morphology and mechanics. ACS APPL. MATER. INTERFACES. 2023; 15:43403–13; doi.org/10.1021/acsami.3c07785

    2021

    [24]  Cho Y., T. Christoff-Tempesta, D-Y. Kim, G. Lamour, and J. Ortony. Domain-selective thermal decomposition within supramolecular nanoribbons. NAT COMMUN; 2021; 12:7340; doi.org/10.1038/s41467-021-27536-6

    [23]Kim D-Y., T. Christoff-Tempesta, G. Lamour, X. Zuo, KH. Ryu, J. Ortony. Morphological
    transitions of a photoswitchable aramid amphiphile nanostructure
    . NANO LETTERS; doi.org/10.1021/acs.nanolett.0c05048

    [22] Christoff-Tempesta T., Y. Cho, DY. Kim, M. Geri, G. Lamour, AJ. Lew, X. Zuo, WR. Lindemann, JH. Ortony. Self-assembly of aramid amphiphiles into ultra-stable nanoribbons and aligned nanoribbon threads. NAT NANOTECHNOL 2021; doi.org/10.1038/s41565-020-00840-w

    2020

    [21] Lamour G., A. Allard, J. Pelta, S. Labdi, M. Lenz, and C. Campillo. Mapping and Modeling the Nanomechanics of Bare and Protein-Coated Lipid Nanotubes. PHYS REV X. 2020; 10:011031-1–17. doi.org/10.1103/PhysRevX.10.011031

    2019

    [20] Nassar R., E. Wong, J. Gsponer, and G. Lamour. Inverse correlation between amyloid stiffness and size. J AM CHEM SOC. 2019; 141:58–61. doi.org/10.1021/jacs.8b10142

    2018

    [19] Nassar R., E. Wong, JM. Bui, CK. Yip, HB. Li, J. Gsponer, and G. Lamour. Mechanical anisotropy in GNNQQNY amyloid crystals. J PHYS CHEM LETT. 2018; 9: 4901–09. doi.org/10.1021/acs.jpclett.8b02027

    [18] Silverman J., E. Gibbs, X. Peng, K. Martens, C. Balducci, J. Wang, M. Yousefi, C.M. Cowan, G. Lamour, et al. A Rational Structured Epitope Defines a Distinct Subclass of Toxic Amyloid-beta Oligomers. ACS CHEM NEUROSCI. 2018; 9:1591–1606. doi.org/10.1021/acschemneuro.7b00469

    2017

    [17] Lamour G., R. Nassar, PHW. Chan, G. Bozkurt, J. Li, JM. Bui, C. Yip, T. Mayor, HB. Li, H. Wu, and J. Gsponer. Mapping the broad structural and mechanical properties of amyloid fibrils. BIOPHYS J. 2017; 112:584–594.  doi.org/10.1016/j.bpj.2016.12.036

    2016

    [16] J. Feng, G. Lamour, R. Xue, MN. Mirvakliki, SG. Hatzikiriakos, J. Xu, HB. Li, S. Wang, X. Lu. Chemical, physical and morphological properties of bacterial biofilms affect survival of encased Campylobacter jejuni F38011 under aerobic stress. INT J FOOD MICROBIOL. 2016; 238:172–182.  doi.org/10.1016/j.ijfoodmicro.2016.09.008

    2015

    [15] Panwar P., G. Lamour, NCW. Mackenzie, H. Yang, F. Ko, HB. Li, D. Brömme. Changes in structural-mechanical properties and degradability of collagen during ageing-associated modifications. J BIOL CHEM. 2015; 290:23291–306.  doi.org/10.1074/jbc.M115.644310

    [14] Lamour G., S. Souès, A. Hamraoui. Substrate-induced PC12 cell differentiation without filopodial, lamellipodial activity or NGF stimulation. MACROMOL BIOSCI. 2015; 15:364–371.  doi.org/10.1002/mabi.201400323

    2014

    [13] Lamour G., JB. Kirkegaard, HB. Li, TPJ. Knowles, and J. Gsponer. Easyworm: an open-source software tool to determine the mechanical properties of worm-like chains. SOURCE CODE BIOL MED. 2014; 9:16.1–16.6.  doi.org/10.1186/1751-0473-9-16

    [12] Kovacic S., L. Samii, G. Lamour, HB. Li, H. Linke, EHC. Bromley, DN. Woolfson, PMG. Curmi, and NR. Forde. Construction and characterization of kilobasepair densely labeled peptide-DNA. BIOMACROMOLECULES. 2014; 15:4065–4072. doi.org/10.1021/bm501109p

    [11] He C., G. Lamour, A. Xiao, J. Gsponer and HB Li. Mechanically Tightening a Protein Slipknot into a Trefoil Knot. J AM CHEM SOC. 2014; 136:11946–11955. doi.org/10.1021/ja503997h

    [10] Lamour G., C. Yip, HB. Li, and J. Gsponer. High intrinsic mechanical flexibility of mouse prion nanofibrils revealed by measurements of axial and radial Young’s moduli. ACS NANO. 2014; 8:3851–3861. doi.org/10.1021/nn5007013

    2013

    [9] Ostapchenko VG., FH. Beraldo, AH. Mohammad, YF. Xie, P. Hirata, AC. Magalhaes, G. Lamour, et al. The prion protein ligand, stress-inducible phosphoprotein I (STI1), regulates amyloid- ; oligomer toxicity. J. NEUROSCI. 2013; 33:16552–16564.  doi.org/10.1523/JNEUROSCI.3214-13.2013

    [8] Cumberworth A. , G. Lamour, M. Babu, and J. Gsponer. Promiscuity as a functional trait: Intrinsically disordered regions as central players of interactomes. BIOCHEM J. 2013; 454:361–369. doi.org/10.1042/BJ20130545

    [7] Panwar P., X. Du, V. Sharma, G. Lamour, M. Castro, HB. Li, and D. Brömme. Effects of cysteine proteases on the structural and mechanical properties of collagen fibers. J BIOL CHEM. 2013; 288:5940–5960. doi.org/10.1074/jbc.M112.419689

    2012

    [6] Li YD., G. Lamour, J. Gsponer, P. Zheng and HB. Li. The molecular mechanism underlying mechanical anisotropy of the protein GB1. BIOPHYS J. 2012; 103:2361–2368.  doi.org/10.1016/j.bpj.2012.10.035

    2011

    [5] Lamour G., S. Souès, and A. Hamraoui. Interplay between long- and short-range interactions drives neuritogenesis on stiff surfaces. J BIOMED MAT RES A. 2011; 99A: 598–606. doi.org/10.1002/jbm.a.33213

    [4] Khorvash M., G. Lamour, and J. Gsponer. Long-time scale fluctuation s of human prion protein determined by restrained MD simulations. BIOCHEMISTRY. 2011; 50: 10191–94.  doi.org/10.1021/bi2012756

    2010

    [3] Lamour G., A. Eftekhari-Bafrooei, E. Borguet, S. Souès, and A. Hamraoui. Neuronal adhesion and differentiation driven by nanoscale surface free-energy gradients. BIOMATERIALS. 2010; 31:3762–3771. doi.org/10.1016/j.biomaterials.2010.01.099

    [2] Lamour G., A. Hamraoui, A. Buvailo, Y. Xing, S. Keuleyan, V. Prakash, A. Eftekhari-Bafrooei, and E. Borguet. Contact angle measurements using a simplified experimental set-up. J CHEM EDUC. 2010; 87:1403–1407.  doi.org/10.1021/ed100468u

    2009

    [1] Lamour G., N. Journiac, S. Souès, S. Bonneau, P. Nassoy, and A. Hamraoui. Influence of surface energy distribution on neuritogenesis. COLLOIDS SURF B. 2009; 72:208–218.  doi.org/10.1016/j.colsurfb.2009.04.006

    Tutorial Lecturer (chargé de TD)

     

    • 2020: Tutorial Lectures/Workshop on Atomic Force Microscopy to Masters' students

                   → Theory, imaging modes, mechanics

    • 2016: Physics classes to Physics students and Biophysics classes to Biology students at the University of Evry (96 h; 3 × 40 students; 1st year undergraduates) 

                   → Mechanics, electricity and optics

    • 2007: Physics classes to medical school students at the Faculty of Medicine, University of Paris Descartes (66 h; 2 × 40 students; 1st year undergraduates)

                   → Diffusion (fluid statics and dynamics), electricity and optics

     

     

    guillaume.lamour [at] univ-evry.fr

    lamour99 [at] hotmail.com

     

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    ORCID = 0000-0002-9331-5532

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